Its key feature is the replacement of one parental antibody's CH1/CL constant region with the T cell receptor (TCR) constant domain, a design aimed at promoting cognate heavy chain (HC)-light chain (LC) pairing. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. LC-missing species, a byproduct frequently associated with bsAb containing sequence engineered Fab arm, is also identified during WuXiBody production.

Nevertheless, WuXiBody's unique design greatly facilitates removal of this type of impurity, which can otherwise be difficult to clear. The results from Capto SP ImpRes and Capto Q polishing steps are summarized in Table 3. In this work, with two concrete cases (WuXiBodies with asymmetric and symmetric designs, respectively), we showed that Capto MMC ImpRes mixed-mode chromatography can effectively remove LC-missing byproducts as well as aggregates, demonstrating that this resin is a powerful tool for WuXiBody purification.Īntibody aggregates Bispecific antibody (bsAb) Capto MMC ImpRes Light chain (LC)-missing byproduct Mixed-mode chromatography WuXiBody.Ĭopyright © 2020 Elsevier Inc. Capto adhere ImpRes in bind-and-elute mode. HiTrap Capto Q ImpRes is a strong anion exchanger prepacked with BioProcess Capto ImpRes media for screening and small-scale protein purifications using ion exchange chromatography (IEX).īecause of poor resolution between the monomer and aggregates, MAb3 could not be purified in flow-through mode on Capto adhere ImpRes, and hence, bind-and-elute mode was evaluated. InertSustainSwift C18 Preparative Columns, Particle Size 5µm, I.D. Capto adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules.Inertsil ODS-4 Capillary Columns, Particle Size 5µm, I.D.High-throughput medium for intermediate and polishing protein purification, excellent choice for process-scale purification. InertSustainSwift C18 Capillary Columns, Particle Size 3µm, I.D. The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere and Capto Q ImpRes. Ultra C8 Columns (USP L7), Particle Size 5 μm, Length 250 mm, ID 4.6 mm, Restek (CAT#: STEM-C-0822-LC) High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional ligands Shop Cytiva HiScreen Capto Cytiva HiScreen Capto Q ImpRes and Capto SP ImpRes Capto SP ImpRes Ionic Capac.: 0.16-0.Capto MMC ImpRes is a chromatographic medium based on a multimodal cation exchange ligand (Fig 1). Inertsil ODS-SP Cartridge Guard Column E, Replacement Cartridge E (2/pk) & Holder Set, Particle Size 3µm, I.D.The ligand constitutes a hydrophobic part, a weak cation exchange group, and groups that can promote hydrogen bonds. Rtx-1 Columns (Fused Silica), Length 15 m, ID 0.32 mm, df 0.25 μm, Temp.Ĭapto MMC ImpRes comprises small ( 40 m) beads relative to the related multimodal cation exchange medium, Capto MMC ( 75 m).Capto Q ImpRes: Strong anion (quaternary amine group) exchange BioProcess medium Capto SP ImpRes: Strong cation (sulfonate group) exchange BioProcess medium.

Inertsil ODS-HL Guard Columns For UHPLC, Replacement Cartridge (2/pk) & Holder Set For UHPLC, Particle Size 3µm, I.D.HiTrap column prepacked with Capto Q ImpRes resin. InertSustainSwift C18 Capillary Columns, Particle Size 5µm, I.D.Designed to offer quality and flexibility in process-scale applications. Scaling up allows for high resolution combined with quick flow rates. Capto Q ImpRes resin couples a quarternary ammonium (Q) strong anion exchanger to a chemically modified, high-flow agarose matrix.

Capto mmc impres